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Based on the quenching effect of emodin on the luminol-myoglobin (Mb) reaction, a sensitive method for the determination of nanogram level emodin by flow injection chemiluminescence (FI-CL) is presented for the first time. It was found that the CL intensity from luminol-Mb system could be inhibited in the presence of emodin, and the decrement of CL intensity was linearly proportional to the logarithm of emodin concentration in the range of 0.5–300ng⋅mL−1   (R = 0.9956) with the detection limit of 0.2ng⋅mL−1 (3σ). At a flow rate of 2.0mL⋅min−1, a complete determination of emodin, including sampling and washing, could be accomplished in 0.5 min with the relative standard deviations (RSDs) of less than 3.5% (n = 5). The proposed method was successfully applied to the determination of emodin in pharmaceutical preparations and human serum samples. The possible CL mechanism of luminol-Mb-emodin reaction was explained.

Determination of Nanogram Quantities of Emodin in Pharmaceutical Preparations and Biofluids by Luminol-Myoglobin Chemiluminescence System