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Braun/murein lipoprotein (Lpp) is involved in inflammatory responses and septic shock. We previously characterized a Δ lpp  mutant of  Yersinia pestis  CO92 and found that this mutant was defective in surviving in macrophages and was attenuated in a mouse inhalation model of plague when compared to the highly virulent wild-type (WT) bacterium. We performed global transcriptional profiling of WT  Y. pestis  and its Δ lpp  mutant using microarrays. The organisms were cultured at 26 and 37 degrees Celsius to simulate the flea vector and mammalian host environments, respectively. Our data revealed vastly different effects of  lpp  mutation on the transcriptomes of  Y. pestis  grown at 37 versus 26°C. While the absence of Lpp resulted mainly in the downregulation of metabolic genes at 26°C, the  Y. pestis  Δ lpp  mutant cultured at 37°C exhibited profound alterations in stress response and virulence genes, compared to WT bacteria. We investigated one of the stress-related genes ( htrA ) downregulated in the Δ lpp  mutant relative to WT  Y. pestis . Indeed, complementation of the Δ lpp  mutant with the  htrA  gene restored intracellular survival of the  Y. pestis  Δ lpp  mutant. Our results support a role for Lpp in  Y. pestis  adaptation to the host environment, possibly via transcriptional activation of  htrA .

comparative and functional genomics

Comparative Global Gene Expression Profiles of Wild-Type<i>Yersinia pestis</i>CO92 and Its Braun Lipoprotein Mutant at Flea and Human Body Temperatures

Comparative Global Gene Expression Profiles of Wild-TypeYersinia pestisCO92 and Its Braun Lipoprotein Mutant at Flea and Human Body Temperatures