Open AccessFemtosecond Time ResolvedInfrared Spectroscopy of theEthylenic Stretch VibrationDuring the all‐Trans to 13‐CisIsomerization ofBacteriorhodopsin
Author(s) -
R. Dziewior,
Karsten Romey,
Rolf Diller
Publication year - 1999
Publication title -
laser chemistry
Language(s) - English
Resource type - Journals
eISSN - 1026-8014
pISSN - 0278-6273
DOI - 10.1155/1999/97059
Subject(s) - chemistry , photochemistry , photoexcitation , photoisomerization , picosecond , ultrafast laser spectroscopy , chromophore , isomerization , bacteriorhodopsin , femtosecond , absorption (acoustics) , absorption spectroscopy , absorption band , vibrational energy relaxation , relaxation (psychology) , infrared , infrared spectroscopy , spectroscopy , atomic physics , optics , excited state , laser , organic chemistry , physics , molecule , membrane , quantum mechanics , biochemistry , psychology , social psychology , catalysis
We report on room temperature infrared transient absorption experiments onBacteriorhodopsin concerning the early picosecond dynamics of the all trans to 13-cisisomerization of the retinal chromophore. The absorption band of the all-transchromophore ethylenic stretch vibration bleaches concomitantly to photoexcitationwhereas new absorption strength is created redshifted within less than 1 ps. the initialbleach recovers partly according to the non unity quantum yield of the photoisomerizationwithin 2 ps. With a time constant of 3ps the low energy absorption band isdownshifled 5–10 cm -1 . Thus, vibrational relaxation and cooling processes appear tohave ended within a few ps and on the time scale of 20 ps a stationary BR-K differencespectrum is obtained.
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