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Angiotensin II (ANG II) stimulates proximal nephron transport via activation of classical protein kinase C (PKC) isoforms. Acute fructose treatment stimulates PKC and dietary fructose enhances ANG II’s ability to stimulate Na + transport, but the mechanisms are unclear. We hypothesized that dietary fructose enhances ANG II’s ability to stimulate renal proximal tubule Na + reabsorption by augmenting PKC-α activation and increases in intracellular Ca 2+ . We measured total and isoform-specific PKC activity, basal and ANG II-stimulated oxygen consumption, a surrogate of Na + reabsorption, and intracellular Ca 2+ in proximal tubules from rats given either 20% fructose in their drinking water (fructose group) or tap water (control group). Total PKC activity was measured by ELISA. PKC-α, PKC-β, and PKC-γ activities were assessed by measuring particulate-to-soluble ratios. Intracelluar Ca 2+ was measured using fura 2. ANG II stimulated total PKC activity by 53 ± 15% in the fructose group but not in the control group (−15 ± 11%, P &lt; 0.002). ANG II stimulated PKC-α by 0.134 ± 0.026 but not in the control group (−0.002 ± 0.020, P &lt; 0.002). ANG II increased PKC-γ activity by 0.008 ± 0.003 in the fructose group but not in the control group ( P &lt; 0.046). ANG II did not stimulate PKC-β in either group. ANG II increased Na + transport by 454 ± 87 nmol·min −1 ·mg protein −1 in fructose group, and the PKC-α/β inhibitor Gö6976 blocked this increase (−96 ± 205 nmol·min −1 ·mg protein −1 , P &lt; 0.045). ANG II increased intracellular Ca 2+ by 148 ± 53 nM in the fructose group but only by 43 ± 10 nM in the control group ( P &lt; 0.035). The intracellular Ca 2+ chelator BAPTA blocked the ANG II-induced increase in Na + transport in the fructose group. We concluded that dietary fructose enhances ANG II’s ability to stimulate renal proximal tubule Na + reabsorption by augmenting PKC-α activation via elevated increases in intacellular Ca 2+ .

Dietary fructose enhances angiotensin II-stimulated Na+transport via activation of PKC-α in renal proximal tubules

Dietary fructose enhances angiotensin II-stimulated Na⁺transport via activation of PKC-α in renal proximal tubules