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Angiotensin II exacerbates oxidative stress in part by increasing superoxide ([Formula: see text]) production by many renal tissues. However, whether it does so in proximal tubules and the source of [Formula: see text] in this segment are unknown. Dietary fructose enhances the stimulatory effect of angiotensin II on proximal tubule Na +  reabsorption, but whether this is true for oxidative stress is unknown. We hypothesized that angiotensin II causes proximal nephron oxidative stress in part by stimulating NADPH oxidase (NOX)4-dependent [Formula: see text] production and decreasing the amount of the antioxidant glutathione, and this is exacerbated by dietary fructose. We measured basal and angiotensin II-stimulated [Formula: see text] production with and without inhibitors, NOX1 and NOX4 expression, and total and reduced glutathione (GSH) in proximal tubules from rats drinking either tap water (control) or 20% fructose. Angiotensin II (10 nM) increased [Formula: see text] production by 113 ± 42 relative light units·mg protein −1 ·s −1  in controls and 401 ± 74 relative light units·mg protein −1 ·s −1  with 20% fructose ( n = 11 for each group, P &lt; 0.05 vs. control). Apocynin and the Nox1/4 inhibitor GKT136901 prevented angiotensin II-induced increases in both groups. NOX4 expression was not different between groups. NOX1 expression was undetectable. Angiotensin II decreased GSH by 1.8 ± 0.8 nmol/mg protein in controls and by 4.2 ± 0.9 nmol/mg protein with 20% fructose ( n = 18 for each group, P &lt; 0.047 vs. control). We conclude that 1) angiotensin II causes oxidative stress in proximal tubules by increasing [Formula: see text] production by NOX4 and decreasing GSH and 2) dietary fructose enhances the ability of angiotensin II to stimulate [Formula: see text] and diminish GSH, thereby exacerbating oxidative stress in this segment.

Angiotensin II-induced superoxide and decreased glutathione in proximal tubules: effect of dietary fructose