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Myocardial tissue in optimal cutting temperature (OCT) fixation and cryostat sectioning was tested as a means of storing and preparing tissue for myofilament function analysis in relation to conventional liquid nitrogen freezing and dissection. Actomyosin interaction, Ca 2+  force activation, and passive compliance were tested. The study concluded that OCT storage and cryostat sectioning do not interfere with the actomyosin cross-bridge dynamics or Ca 2+  activation but that absolute tension values suffer and may not be investigated by this method.

Optimal cutting temperature medium embedding and cryostat sectioning are valid for cardiac myofilament function assessment