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Bacterial quorum sensing can be engineered with a view to the design ofbiotechnological applications based on their intrinsic role as a means ofcommunication. We propose the creation of a positive feedback loop that willpromote the emission of a superfolded green fluorescence protein from abacterial population that will flow through hydrogel, which is used toencapsulate the cells. These engineered cells are heretofore referred to asbio-nanomachine transmitters and we show that for lower values of diffusioncoefficient, a higher molecular output signal power can be produced, whichsupports the use of engineered bacteria contained within hydrogels formolecular communications systems. In addition, our wet lab results show thepropagation of the molecular output signal, proving the feasibility ofengineering a positive feedback loop to create a bio-nanomachine transmitterthat can be used for biosensing applications.

Hydrogel-based Bio-nanomachine Transmitters for Bacterial Molecular Communications