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In living cells, molecular motors create activity that enhances the diffusionof particles throughout the cytoplasm, and not just ones attached to themotors. We demonstrate initial steps toward creating artificial cells thatmimic this phenomenon. Our system consists of active, Pt-coated Janus particlesand passive tracers confined to emulsion droplets. We track the motion of boththe active particles and passive tracers in a hydrogen peroxide solution, whichserves as the fuel to drive the motion. We first show that correcting for bulktranslational and rotational motion of the droplets induced by bubble formationis necessary to accurately track the particles. After drift correction, we findthat the active particles show enhanced diffusion in the interior of thedroplets and are not captured by the droplet interface. At the particle andhydrogen peroxide concentrations we use, we observe little coupling between theactive and passive particles. We discuss the possible reasons for lack ofcoupling and describe ways to improve the system to more effectively mimiccytoplasmic activity.

Active colloidal particles in emulsion droplets: A model system for the cytoplasm