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We assessed the accuracy of the FraserLee, biological-intercept, and Weisberg back-calculation models to estimate growth from otoliths and scales of laboratory-reared juvenile bluegill × green sunfish hybrids (Lepomis macrochirus × Lepomis cyanellus). Hybrid sunfish were injected three times with oxytetracycline hydrochloride at 90-day intervals to mark bony structures, creating simulated annuli for model validation. Back-calculated lengths (BCLs) with otoliths were generally less accurate than scales for all three models. Errors ranged from 8.2 to 7.8% for the FraserLee model, from 8.0 to 8.3% for the biological-intercept model, and from 6.5 to 14.3% for the Weisberg model. For all three models, there was no significant difference in BCLs using left or right otoliths, and BCLs using the FraserLee and biological-intercept models were not significantly different from each other. In contrast with otoliths, all three models produced accurate BCLs from scales; errors ranged from 4.3 to 0.1%. For juveni...

canadian journal of fisheries and aquatic sciences

Validation of three back-calculation models by using multiple oxytetracycline marks formed in the otoliths and scales of bluegill × green sunfish hybrids