English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Zendy Open

Presents the pdf analysis as premium feature

PDF Analysis

Insights

Presents the summarisation as premium feature

Summarisation

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the zaia usage as premium feature

ZAIA

Zendy Tools

Zendy Plus

Presents the access of premium content as premium feature

Premium Content

The spread of  Klebsiella pneumoniae  carbapenemase (KPC)-producing  Enterobacterales  is a public health concern. KPC-encoding  bla  KPC  is predominantly spread by strains of a particular phylogenetic lineage, clonal group 258, but can also be spread by horizontal transfer of  bla  KPC -carrying plasmids. Here, we report the transfer of a  bla  KPC-2 -harboring plasmid via mobilization from  K. pneumoniae  to  Citrobacter freundii  complex and  Morganella morganii  strains in a single patient. We performed draft whole-genome sequencing to analyze 20 carbapenemase-producing  Enterobacterales  strains (15 of  K. pneumoniae , two of  C. freundii  complex, and three of  M. morganii ) and all  K. pneumoniae  strains using MiSeq and/or MinION isolated from a patient who was hospitalized in New York and Montreal before returning to Japan. All strains harbored  bla  KPC-2 -containing Tn 4401a . The 15  K. pneumoniae  strains each belonged to sequence type 258 and harbored a Tn 4401a -carrying multireplicon-type plasmid, IncN and IncR (IncN+R). Three of these  K. pneumoniae  strains also possessed a Tn 4401a -carrying ColRNAI plasmid, suggesting that Tn 4401a  underwent interplasmid transposition. Of these three ColRNAI plasmids, two and one were identical to plasmids harbored by two  Citrobacter europaeus  and three  M. morganii  strains, respectively. The Tn 4401a -carrying ColRNAI plasmids were each 23,753 bp long and incapable of conjugal transfer via their own genes alone, but they mobilized during the conjugal transfer of Tn 4401a -carrying IncN+R plasmids in  K. pneumoniae . Interplasmid transposition of Tn 4401a  from an IncN+R plasmid to a ColRNAI plasmid in  K. pneumoniae  and mobilization of Tn 4401a -carrying ColRNAI plasmids contributed to the acquisition of  bla  KPC-2  in  C. europaeus  and  M. morganii .   IMPORTANCE  Plasmid transfer plays an important role in the interspecies spread of carbapenemase genes, including the  Klebsiella pneumoniae  carbapenemase (KPC)-coding gene,  bla  KPC . We conducted whole-genome sequencing (WGS) analysis and transmission experiments to analyze  bla  KPC-2 -carrying mobile genetic elements (MGEs) between the  bla  KPC-2 -harboring  K. pneumoniae ,  Citrobacter europaeus , and  Morganella morganii  strains isolated from a single patient.  bla  KPC-2  was contained within an MGE, Tn 4401a . WGS of  bla  KPC-2 -carrying  K. pneumoniae ,  C. europaeus , and  M. morganii  strains isolated from one patient revealed that Tn 4401a -carrying ColRNAI plasmids were generated by plasmid-to-plasmid transfer of Tn 4401a  from a multireplicon-type IncN and IncR (IncN+R) plasmid in  K. pneumoniae  strains. Tn 4401a -carrying ColRNAI plasmids were incapable of conjugal transfer in  C. europaeus  and  M. morganii  but mobilized from  K. pneumoniae  to a recipient  Escherichia coli  strain during the conjugal transfer of Tn 4401a -carrying IncN+R plasmid. Therefore, Tn 4401a -carrying ColRNAI plasmids contributed to the acquisition of  bla  KPC-2  in  C. europaeus  and  M. morganii .

msphere

Molecular Analysis of bla KPC-2 -Harboring Plasmids: Tn 4401a Interplasmid Transposition and Tn 4401a -Carrying ColRNAI Plasmid Mobilization from Klebsiella pneumoniae to Citrobacter europaeus and Morganella morganii in a Single Patient