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We identified a polyadenylated RNA species which contains the origin of human mitochondrial DNA light-strand synthesis and the surrounding complementary sequences of the four light-strand-encoded tRNAs. This RNA (RNA 9L) is probably derived from the leader portion of RNA 6 which is excised during the formation of the mature cytochrome c oxidase subunit mRNA (RNA 9). The high degree of secondary structure of this RNA is presumably responsible for its anomalous electrophoretic behavior in denaturing polyacrylamide gels.

molecular and cellular biology

The Excised Leader of Human Cytochrome <i>c</i> Oxidase Subunit I mRNA which Contains the Origin of Mitochondrial DNA Light-Strand Synthesis Accumulates in Mitochondria and is Polyadenylated

The Excised Leader of Human Cytochrome c Oxidase Subunit I mRNA which Contains the Origin of Mitochondrial DNA Light-Strand Synthesis Accumulates in Mitochondria and is Polyadenylated