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Bacteriophage TSP-1 was isolated from soil in a search for phage which would form plaques on Bacillus subtilis W168 at 53 C. It forms clear plaques only at temperatures from 50 to 55 C. Approximately 95% of the free phage adsorb after 2 min at 53 C. The lytic cycle is between 55 and 60 min long with a burst size of about 55 particles per infected bacterium. The phage was shown to contain double-stranded deoxyribonucleic acid with a base composition of 44.7% guanine plus cytosine. This deoxyribonucleic acid does not contain a base analogue for thymine and has a molecular weight estimated at 56 x 10(6) daltons.

A Bacteriophage of Bacillus subtilis Which Forms Plaques Only at Temperatures Above 50 C I. Physical and Chemical Characteristics of TSP-1