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Mutants in T4 genes 46 and 47 exhibit early cessation of deoxyribonucleic acid (DNA) synthesis (“DNA arrest”) and decreased synthesis of late proteins and phage. In addition, mutants in genes 46 and 47 fail to degrade host DNA to acidsoluble products. It is shown here that this complex phenotype can be partially suppressed by mutation of a T4 gene external to genes 46 and 47 which has been nameddas for “D NAa rrests uppressor.” Thedas mutations were discovered as third-site mutations in spontaneous pseudorevertants of [46, 47] mutants; the pseudorevertants make small plaques onEscherichia coli B, whereas [46, 47] mutants make none. The [das , 46, 47] triple mutant exhibits increased DNA, late protein, and viable phage production compared to the double mutant [46, 47]. The [das , 46, 47] mutant also degrades more of the host DNA to acid-soluble products than does the [46, 47] mutant. The suppressor effect of thedas mutation appears to be gene-specific: it suppresses bothamber and temperature-sensitive mutations in genes 46 and 47 and does not suppressamber mutations in any of the other genes tested. The [das ] single mutants make normal-sized plaques onE. coli B and exhibit nearly normal host DNA degradation, DNA synthesis, late protein synthesis, and viable phage production. Thedas mutations either define a new gene between genes 33 and 34 or are special mutations within gene 33.

Specific Suppression of Mutations in Genes 46 and 47 by das , a New Class of Mutations in Bacteriophage T4D