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Influenza virus endonuclease activity was studied in vitro with model virion RNA (vRNA) and cRNA molecules. We show that endonuclease activity can be partially rescued by transplanting vRNA-like promoter features into the model cRNA promoter. This study defines three distinctive features within the vRNA promoter--absent in the cRNA promoter--that are required for endonuclease cleavage.

journal of virology

Differential Activation of Influenza A Virus Endonuclease Activity Is Dependent on Multiple Sequence Differences between the Virion RNA and cRNA Promoters