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In this study, Dicentrarchus labrax encephalitis virus (DlEV), which causes sea bass encephalitis, was propagated in cell culture, thus allowing study of its lytic cycle. DlEV infection of mammalian and fish cells induced different patterns of expression of capsid proteins, which were assembled as virus-like particles, accumulating in the cytoplasm either as diffuse masses or in vesicles, as shown by electron microscopy. These particles correspond to virions, as shown by their ability to induce secondary infection. Fish cells proved to be more permissive for DlEV than mammalian cells, although virus yield remained low. RNA analysis of infected sea bass cells revealed DlEV RNA3, in addition to genomic RNA1 and RNA2, and the presence of the RNA2 minus strand, thus demonstrating the replication of the DlEV genome. In addition, DlEV RNA-dependent RNA polymerase was associated with mature virions even after purification by a CsCl gradient, but it was dissociated when capsids were destabilized. In addition to providing more information about the relatedness of DlEV to the members of the family Nodaviridae, this study shows that fish nodaviruses may not be able to infect as wide a variety of cells as insect nodaviruses can.

Fish nodavirus lytic cycle and semipermissive expression in mammalian and fish cell cultures