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The nucleotide sequence of the five regions of homologous DNA in the genome ofAutographa californica nuclear polyhedrosis virus DNA was determined. The homology of repeated sequences within a region was 65 to 87%, and the consensus sequences for each region were 88% homologous to each other. Sequences proximal to theEco RI sites were most conserved, while the distal sequences were least conserved. TheEco RI sites formed the core of a 28-base-pair imperfect inverted repeat. All homologous regions functioned as enhancers in a transient expression assay. A singleEco RI minifragment located betweenEco RI-Q and -L enhanced the expression of 39CAT as efficiently as the regions containing numerousEco RI repeats did.

Complete Sequence and Enhancer Function of the Homologous DNA Regions of Autographa californica Nuclear Polyhedrosis Virus