English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

On the basis of earlier findings showing that H5hr1 (hr1) is cold sensitive for transformation, a series of mutants were constructed so that they contained deletions or insertions in different sites of early region 1a (E1a) to ascertain: (i) whether the cold-sensitive phenotype of hr1 was the result of the identified single-base pair deletion of nucleotide 1,055 or due to a missense mutation at another site and (ii) what region and how much of the E1a 51-kilodalton protein is actually required to produce cell transformation. A mutant, H5dl101 (dl101), was constructed to contain a 5-base pair deletion of nucleotides 1,008 to 1,012, which produced a frameshift and a subsequent stop codon at nucleotide 1,241. This mutant, which should encode a truncated 33-kilodalton protein in place of the wild-type 51-kilodalton protein, had a cold-sensitive phenotype for transformation essentially identical to hr1. Consonant with this finding, a mutant (H5in106) engineered to contain a 16-base pair insertion initiated after nucleotide 1,009, with a stop codon beginning at the newly inserted nucleotide 1,013, also had a cold-sensitive phenotype like hr1 and dl101. It is striking, however, that a mutant (H5dl105) with a 69-base pair deletion beginning at nucleotide 1,003, and having a stop codon at nucleotide 1,544, was totally defective for transformation at any temperature. Transfection studies with plasmids containing the E1a or E1a and E1b genes of sub309, hr1, and dl101 further revealed that the cold-sensitive transformation phenotype observed could be exhibited in the absence of viral E1b gene expression.

Deletion and insertion mutations in early region 1a of type 5 adenovirus that produce cold-sensitive or defective phenotypes for transformation