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Black beetle virus (BBV), one of a recently discovered class of viruses with a bipartite genome, multiplied readily in Schneider's line 1 ofDrosophila cells. Virus yields, on the order of 100 mg per liter of culture, were unusually high and represented some 20% of the total cell protein within 3 days after infection. A derivative subline of theseDrosophila cells was found to be resistant to infection by BBV. These resistant cells were also found to carry small amounts of BBV-related particles, possibly a maturation-defective form of BBV.

journal of virology

Black Beetle Virus: Propagation in <i>Drosophila</i> Line 1 Cells and an Infection-Resistant Subline Carrying Endogenous Black Beetle Virus-Related Particles

Black Beetle Virus: Propagation in Drosophila Line 1 Cells and an Infection-Resistant Subline Carrying Endogenous Black Beetle Virus-Related Particles