English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

The genome of the simian virus 40 (SV40) temperature-sensitive (ts ) mutanttsD202 rescued by passage on transformed permissive monkey lines (see accompanying paper [Y. Gluzman et al., J. Virol.24 :534-540, 1977]) was analyzed by restriction endonuclease cleavage mapping to obtain biochemical evidence that the rescue of thets phenotype results from recombination with the resident SV40 genome of the transformed cell. It was demonstrated that the endonuclease R·Hae III cleavage site, which is located at 0.9 map unit in the standard viral genome (and which is in the proximity of the known map position of thetsD lesion), is missing in the DNAs of the parentaltsD202 virus and of three independent revertants oftsD202. In contrast, this cleavage site was shown to be present in the DNAs of four out of five independently derived rescuedD202 populations and in the DNA of the SV40 strain, 777, used to transform the monkey cells. Comparison of the endonuclease R·Hin (II + III) cleavage patterns of SV40 strain 777 DNA andtsD202 DNA revealed differences in the electrophoretic mobilities ofHin fragments A, B, and F. However, the correspondingHin fragments from all four rescuedD202 genomes were identical in their mobilities to those oftsD202 DNA, indicating that these regions of the rescuedD202 genome are characteristic of thetsD202 parent. We conclude, therefore, that the genome of the rescuedD202 virus is a true recombinant, since it contains restriction endonuclease cleavage sites characteristic of both parents, the endogenous resident SV40 genome of the transformed monkey cells and the exogenoustsD202 mutant.

Recombination Between Endogenous and Exogenous Simian Virus 40 Genes. II. Biochemical Evidence for Genetic Exchange