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We have studied the properties of presumptive point mutants in the D2a region of bacteriophage T4. Dominance tests showed that the D2a mutation was recessive to the wild-type allele. The mutations were shown to map in the D2a region by complementation againstr II deletions. The D2a mutations were also located between gene 52 andr IIB by two- and three-factor crosses. The mutants are located at at least two distinct loci in the D2a region. The point mutants grow normally on all hosts tested and none of the mutants makes T4 endonuclease IV. We propose the name “den B” for the D2a locus.

Point Mutants in the D2a Region of Bacteriophage T4 Fail to Induce T4 Endonuclease IV