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A 10- to 15-min derepression of a λ prophage in a Gal− recipient during early infection with a transducing lysate of coliphage T1am will cause an increase in the efficiency of transduction of Gal+ . An increase in the efficiency of transduction occurs when the donor is either nonlysogenic or lysogenic for λ; the increase is blocked by rifampin or chloramphenicol. With strain R901 it has been shown that efficient transduction can be blocked by treatment with rifampin after all chloramphenicol-sensitive steps have occurred.

Transduction of Gal + by Coliphage T1 III. Requirement for Transcription and Translation in Recipient Cells

Transduction of Gal ⁺ by Coliphage T1 III. Requirement for Transcription and Translation in Recipient Cells