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Classa and classb 30 to 40S RNA subunits obtained by heat dissociation from the 60 to 70S RNA complex of avian tumor viruses were compared with several RNA standards by electrophoresis in formamide-polyacrylamide gels. Classa RNA was found to have a lower electrophoretic mobility and hence probably a higher molecular weight than classb RNA. The absolute molecular weight of classa andb RNA could not be determined with accuracy, because the relationship between logarithm of molecular weight and mobility of the RNA standards was not linear. The size of classa RNA fell into the range of 2.4 × 106 to 3.4 × 106 daltons and that of classb into the range of 2.2 × 106 to 2.9 × 106 daltons, depending on the standards used. The possible biological significance of this difference is discussed.

Gel Electrophoresis of Avian Leukosis and Sarcoma Viral RNA in Formamide: Comparison with Other Viral and Cellular RNA Species