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By inhibiting techniques using indirect immunofluorescence tests and indirect immunoelectron microscopy, the G(Gross) soluble antigens (GSA) in the body fluids of AKR and C58 mice, which have a high incidence of spontaneous leukemia, were classified according to the known specificity of G antigens in the murine Gross leukemia system. GSA existing in the plasma of nonleukemic and leukemic AKR mice and in the ascitic fluid of transplanted AKR spontaneous leukemia K36 showed the several specificities corresponding to G cell surface antigens, GCSAa, b, and c, and type-specific and group-specific viral envelope antigens, tsVEA and gsVEA, respectively. However, the plasma of nonleukemic C58 mice lacks GSAc, which can be recognized by the G-typing mouse serum. GSA corresponding to G(IX) antigen was not detected in the body fluids.

Wild-Type Gross Leukemia Virus: Classification of Soluble Antigens (GSA)