Open AccessDetermination of Sequences Required for Human Endogenous Retrovirus K Transduction and Its Recognition by Foreign Retroviral Virions
Author(s) -
Otto Erlwein,
Nathan P. Sweeney,
Raffaele de Leon,
Gillian Wills,
Mark J. Robinson,
Myra O. McClure
Publication year - 2015
Publication title -
journal of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.617
H-Index - 292
eISSN - 1070-6321
pISSN - 0022-538X
DOI - 10.1128/jvi.02731-15
Subject(s) - biology , transduction (biophysics) , retrovirus , endogenous retrovirus , murine leukemia virus , virology , genome , gene , genetics , endogeny , feline leukemia virus , virus , group specific antigen , computational biology , endocrinology , biochemistry
Sequences necessary for transduction of human endogenous retrovirus (HERV)-Kcon, a consensus of the HERV-K(HML-2) family, were analyzed and found to reside in the leader/gag region. They act in an orientation-dependent way and consist of at least two sites working together. Having defined these sequences, we exploited this information to produce a simple system to investigate to what extent virions of HERV-Kcon, murine leukemia virus, and HIV-1 have the ability to transduce each other's genomes, leading to potential contamination of gene therapy vectors.
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