Open AccessModulation of Flavivirus Population Diversity by RNA Interference
Author(s) -
Doug E. Brackney,
Erin E. Schirtzinger,
Thomas Harrison,
Gregory D. Ebel,
Kathryn A. Hanley
Publication year - 2015
Publication title -
journal of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.617
H-Index - 292
eISSN - 1070-6321
pISSN - 0022-538X
DOI - 10.1128/jvi.02612-14
Subject(s) - biology , viral quasispecies , rna interference , flavivirus , virology , rna , innate immune system , population , virus , rna silencing , interference (communication) , genetics , immune system , gene , hepatitis c virus , engineering , channel (broadcasting) , demography , electrical engineering , sociology
To test the hypothesis that RNA interference (RNAi) imposes diversifying selection on RNA virus genomes, we quantified West Nile virus (WNV) quasispecies diversity after passage in Drosophila cells in which RNAi was left intact, depleted, or stimulated against WNV. As predicted, WNV diversity was significantly lower in RNAi-depleted cells and significantly greater in RNAi-stimulated cells relative to that in controls. These findings reveal that an innate immune defense can shape viral population structure.
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