Open AccessAdaptation of an R5 Simian-Human Immunodeficiency Virus Encoding an HIV Clade A Envelope with or without Ablation of Adaptive Host Immunity: Differential Selection of Viral Mutants
Author(s) -
Mingkui Zhou,
Michael Humbert,
Muhammad M. Mukhtar,
Hanna B. Scinto,
Hemant K. Vyas,
Samir K. Lakhashe,
Nagadenahalli B. Siddappa,
Gregor Maurer,
Swati Thorat,
Joshua O. Owuor,
Zhao Lai,
Yidong Chen,
Anthony Griffiths,
AgnèsLaurence Chenine,
Sanjeev Gumber,
François Villinger,
David C. Montefiori,
Ruth M. Ruprecht
Publication year - 2019
Publication title -
journal of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.617
H-Index - 292
eISSN - 1070-6321
pISSN - 0022-538X
DOI - 10.1128/jvi.02267-18
Subject(s) - biology , virology , adaptation (eye) , clade , simian immunodeficiency virus , human immunodeficiency virus (hiv) , immunity , host (biology) , genetics , viral envelope , gene , viral replication , virus , immune system , phylogenetics , neuroscience
In this study, we constructed a simian-human immunodeficiency virus carrying an R5 Kenyan HIV-1 clade Aenv (SHIV-A). To bypass host immunity, SHIV-A was rapidly passaged in naive macaques or animals depleted of both CD8+ and B cells. Next-generation sequencing identified different mutations that resulted from optimization of viral replicative fitness either in the absence of adaptive immunity or due to pressure from adaptive immune responses.
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