Open AccessNovel Multiplex PCR Assay for Detection of the Staphylococcal Virulence Marker Panton-Valentine Leukocidin Genes and Simultaneous Discrimination of Methicillin-Susceptible from -Resistant Staphylococci
Author(s) -
Jo-Ann McClure,
John Conly,
Vincent K. N. Lau,
Sameer Elsayed,
Thomas Louie,
Wendy Hutchins,
Kunyan Zhang
Publication year - 2006
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.44.3.1141-1144.2006
Subject(s) - panton–valentine leukocidin , leukocidin , virulence , multiplex , microbiology and biotechnology , staphylococcus aureus , multiplex polymerase chain reaction , biology , virology , staphylococcal infections , micrococcaceae , gene , methicillin resistant staphylococcus aureus , polymerase chain reaction , bacteria , genetics
We developed a new multiplex PCR assay for detection of Panton-Valentine leukocidin virulence genes and simultaneous discrimination of methicillin-susceptible from -resistant staphylococci. This assay is simple, rapid, and accurate and offers the potential for prompt detection of newly emerging community-associated methicillin-resistantStaphylococcus aureus .