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Malaria remains the most dangerous tropical disease for the French troops deployed overseas. In 2003, 768 cases in the French army were declared along with an increase of incidence of 400% in comparison with 2002, mainly due to the Licorne peacekeeping operation in Ivory Coast. In order to ensure fast diagnosis in the field, a rapid immunochromatographic test (NOW malaria test; Binax, Portland, Oreg.) has been provided to military doctors. This test detects the Plasmodium falciparum-specific HRP2 anti- gen and a panmalarial aldolase common to all species. The assay is sensitive for the detection of P. falciparum (1) and Plasmodium vivax (2) but, in our experience, is poorly sensitive for the detec- tion of Plasmodium ovale, a species involved in cases of malaria among the French troops in western Africa. Between November 2002 and August 2004, 114 samples from patients presenting a malaria attack were analyzed in the laboratory of the French military hospital of Begin (Saint- Mande, France). The Plasmodium species identified were P. falciparum (n 93), P. ovale (n 12), P. vivax (n 9), and P. malariae (n 1). For each specimen, thin and thick blood films, the Binax NOW malaria test, and a specific SYBR green real-time PCR using the Lightcycler instrument (Roche Diag- nostics, Meylan, France) were used for diagnosis. This tech- nique is considered the "gold standard" in our laboratory. Sequences used for the design of primers were the 18S RNA genes of the four species (Table 1), which have been previously validated (data not shown). Thin and thick blood films were stained with a rapid coloration set (Diff-Quick; Dade Behring, Newark, Del.) and examined by two experienced microscopists during 20 min. The rapid immunochromatographic test was used according to the manufacturer's recommendations. Among the 22 patients infected with non-P. falciparum spe- cies, 9 were positive for P. vivax, 12 were positive for P. ovale, and 1 was positive for P. malariae by microscopic examination (Table 1). PCR was used to detect all the infections and con- firm all microscopic identifications. The Binax NOW malaria test detected all cases of P. vivax infection (9 of 9) but only 3 of the 12 cases of P. ovale infection (25%). The test was positive in the only case of P. malariae infection. With P. falciparum ,8 9 cases were positive, and two false positives and one false neg- ative were found. Considering these data, it seems that the Binax NOW ma- laria test is not reliable for the detection of P. ovale infection. This has been previously described in a study including nine cases of P. ovale infection (2). The inability of the rapid im- munochromatographic test to detect P. ovale has been also observed with the ICT Malaria P.f/P.v test (3, 4). The main explanation for the failure of the assay was low parasite den- sity, but in this study all infections due to P. ovale were de- tected by microscopic examination. The inaccuracy could be due to low production of the aldolase by P. ovale or, as sup- posed by Mason et al., to regional variations in the genetic determinants of ICT panmalarial antigen (5). In case of sus- picion of malaria challenge, the diagnosis of infection with P. ovale must not be elicited, and blood smear examination re- mains necessary for the elimination of the diagnosis.

Ineffectiveness of the Binax NOW Malaria Test for Diagnosis of Plasmodium ovale Malaria