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A TaqMan allelic-discrimination assay designed around a synonymous single-nucleotide polymorphism was used to genotypeBurkholderia pseudomallei andBurkholderia mallei isolates. The assay rapidly identifies and discriminates between these two highly pathogenic bacteria and does not cross-react with genetic near neighbors, such asBurkholderia thailandensis andBurkholderia cepacia .

Use of a Real-Time PCR TaqMan Assay for Rapid Identification and Differentiation of Burkholderia pseudomallei and Burkholderia mallei