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We describe a case of Gemella bergeriae endocarditis in a patient with a bicuspid aortic valve. Diagnosis was confirmed by sequencing of 16S rRNA genes in heart valve tissue. This is the first report of Gemella endocarditis confirmed by molecular detection of bacterial genes in heart valve tissue.

Gemella bergeriae Endocarditis Diagnosed by Sequencing of rRNA Genes in Heart Valve Tissue