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Three clinical strains of enterohemorrhagic Escherichia coli O157:H7 which were subcultured repeatedly or stored at room temperature over a 25-week period showed appreciable variations in their pulsed-field gel electrophoresis fragment patterns. The variations could be explained by a couple of spontaneous genetic events at most and thus did not invalidate the genetic lineage of the strains.

journal of clinical microbiology

Effects of Repeated Subculturing and Prolonged Storage at Room Temperature of Enterohemorrhagic <i>Escherichia coli</i> O157:H7 on Pulsed-Field Gel Electrophoresis Profiles

Effects of Repeated Subculturing and Prolonged Storage at Room Temperature of Enterohemorrhagic Escherichia coli O157:H7 on Pulsed-Field Gel Electrophoresis Profiles