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Culture of autologous CD4 lymphocytes from peripheral blood mononuclear cells compared favorably with two other methods for the measurement of cell-associated human immunodeficiency virus type 1 (HIV-1). For subjects with undetectable HIV-1 RNA levels in plasma, there was a 10,000-fold range of cell-associated virus detected. This method provides a simple and reproducible means for monitoring cell-associated HIV-1.

journal of clinical microbiology

Marked Differences in Quantity of Infectious Human Immunodeficiency Virus Type 1 Detected in Persons with Controlled Plasma Viremia by a Simple Enhanced Culture Method