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We evaluated the sensitivity and specificity of a PCR-based qualitative test for the rapid diagnosis ofMycobacterium avium-M. intracellulare complex (MAC) bacteremia in patients with AIDS disease. Eleven subjects with newly culture-proven MAC bacteremia had the following tests performed at biweekly intervals during the first 8 weeks of therapy: blood culture,Mycobacterium -specific PCR, and quantitative human immunodeficiency virus (HIV) viral-load testing.Mycobacterium genus-specific biotinylated primers were used to amplify a sequence of approximately 582 nucleotides within the 16S rRNA genes ofM. avium andM. intracellulare . Detection of the amplified product was performed with an oligonucleotide probe-coated microwell plate combined with an avidin-horseradish peroxidase–tetramethylbenzidine conjugate-substrate system. While not as sensitive as BACTEC culture, PCR detected 17 of 18 specimens which grew ≥40 organisms/ml (94.4% sensitivity) and 9 of 16 specimens which grew ≤40 organisms/ml (56.3% sensitivity). No clear change in HIV viremia occurred in response to successful treatment of patients’ MAC bacteremia. Use of the PCR test allowed detection of MAC bacteremia in 1 day, with a sensitivity similar to those of quantitative blood culture techniques, and it may prove useful for rapid screening of suspected cases. HIV viremia was unaffected by 8 weeks of MAC therapy.

journal of clinical microbiology

Use of PCR in Detection of <i>Mycobacterium avium</i> Complex (MAC) Bacteremia: Sensitivity of the Assay and Effect of Treatment for MAC Infection on Concentrations of Human Immunodeficiency Virus in Plasma

Use of PCR in Detection of Mycobacterium avium Complex (MAC) Bacteremia: Sensitivity of the Assay and Effect of Treatment for MAC Infection on Concentrations of Human Immunodeficiency Virus in Plasma