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A Chlamydia trachomatis D* serovariant strain was found to be mixed with an F serovar strain in a clinical specimen. By using a monoclonal antibody which neutralizes serovar F infectivity in hamster kidney cells, the D* variant strain was enriched until it could be cloned by limiting dilution. This newly described neutralization enrichment procedure can be used to purify a C. trachomatis serovar present in small numbers in a mixed culture or, potentially, to identify nonneutralizable mutants.

Purification of Chlamydia trachomatis strains in mixed infection by monoclonal antibody neutralization