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We compared traditional complement fixation (CF) with a new passive agglutination method, the Seradyn Color Vue (SCV) test (Seradyn, Indianapolis, Ind.), for detection of Mycoplasma pneumoniae antibodies in 170 stored serum samples. The SCV test was 90% sensitive in identifying as positive 27 of 30 CF high-titer (&gt; or = 1:64) serum samples and 100% specific in identifying as negative 134 of 134 CF low-titer (&lt; or = 1:32) or negative (&lt; 1:8) serum samples. The SCV test was technically undemanding, and it required no expensive equipment.

Comparison of the Seradyn Color Vue passive agglutination test and complement fixation for detection of Mycoplasma pneumoniae antibodies