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All biotyped strains of Corynebacterium diphtheriae from the American Type Culture Collection (ATCC) were compared for morphology and biochemical reactions. Biotypes of all gravis strains and most mitis strains were confirmed, but intermedius strains were found to be misclassified. New lipid-dependent intermedius strains have been deposited with the ATCC.

Laboratory review of reference strains of Corynebacterium diphtheriae indicates mistyped intermedius strains