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The isolation and identification of an adventitious viral agent, infectious bovine rhinotracheitis virus, in one lot of A549 cells from a commercial supplier is described in this report. The presence of infectious bovine rhinotracheitis virus in A549 cells was unexpected and has caused problems in the diagnosis of infections in clinical specimens in three laboratories.

journal of clinical microbiology

An adventitious viral contaminant in commercially supplied A549 cells: identification of infectious bovine rhinotracheitis virus and its impact on diagnosis of infection in clinical specimens