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Twenty-six serotypes of Xanthomonas maltophilia were defined by using 15 antisera described by Hugh and Ryschenkow (R. Hugh and E. Ryschenkow, J. Gen. Microbiol. 26:123-132, 1961) and 11 new antisera. The antisera were prepared by immunizing rabbits with bacterial strains heated at 100 degrees C for 2 h. Twelve antisera required adsorptions with cross-reacting heterologous immunizing strains. We tested 275 clinical and environmental strains of X. maltophilia with 26 antisera by the slide agglutination technique. A total of 259 (94.2%) strains were typeable, with 137 (49.8%) strains agglutinating in three antisera.

Serological classification of Xanthomonas maltophilia (Pseudomonas maltophilia) based on heat-stable O antigens