English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

2',5'-Oligoadenylate [2',5'-oligo(A)] was separated from 14C-labeled nucleosides produced in the 2',5'-oligo(A) synthetase assay by using 100-microliters columns of Dowex 1. No detectable nucleoside remained on the column after elution with 20 column volumes of water, whereas less than 1% of oligonucleotides were eluted from the column. At least 99% of oligonucleotides were eluted from the column with 1 M NaCl, pH 2. The major product had properties consistent with 2',5'-oligo(A). Exposure to alpha-1 bovine interferon (IFN) caused an increase in cellular 2',5'-oligo(A) synthetase activity which was proportional to the concentration of IFN in the medium up to 10(4) U of IFN per ml and then leveled off at about 15 X control activity. Under the assay conditions used, 2',5'-oligo(A) synthetase activity was directly proportional to the amount of cell extract over a 10-fold range. Cattle inoculated with IBR/BVD/PI-3 modified live virus vaccine showed an increase in 2',5'-oligo(A) synthetase activity in peripheral blood mononuclear leukocytes which persisted for at least 3 days postvaccination. Intramuscular injection of cattle with IFN caused a similar increase in 2',5'-oligo(A) synthetase activity. Changes in 2',5'-oligo(A) synthetase activity should be of value in (i) assessing the response of cattle to experimental viral infections or inoculations with viral vaccines or IFN or (ii) indicating a possible viral etiology in disease.

Induction and measurement of 2',5'-oligoadenylate synthetase in Madin-Darby bovine kidney cells and in cattle