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An automated enzyme-linked immunosorbent assay detecting specific immunoglobulin M in infections with Leptospira interrogans serovar hardjo was evaluated on 69 patients. The test was sensitive and simple to perform, requiring a single dilution of test serum, with data expressed as units of antibody activity interpolated from a reference serum pool.

Enzyme-linked immunosorbent assay for determining specific immunoglobulin M in infections caused by Leptospira interrogans serovar hardjo