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Transgrow bottles with medium containing ammonium bicarbonate and Transgrow bottles gassed with 10% carbon dioxide performed equally well in detecting Neisseria gonorrhoeae in 434 clinical specimens. It appears that incorporation of ammonium bicarbonate into the medium increased the efficiency of the manufacturing process while maintaining the effectiveness of the medium.

Ammonium bicarbonate as a replacement for carbon dioxide in Transgrow bottles for primary isolation of Neisseria gonorrhoeae