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A simple and rapid method has been developed for the separation of serum immunoglobulin M (IgM) and IgG. CM Bio-Gel A chromatography was used in the technique, which resulted in an IgM-rich fraction containing 31% of the original serum IgM and less than 2% of the serum IgG. The procedure was used to detect masked IgM antibodies in patients suspected of having Toxoplasma gondii infections.

Serological diagnosis of Toxoplasma gondii infections by rapid separation of serum immunoglobulins M and G with CM Bio-Gel A