Open AccessA Molecular Approach to Identification and Profiling of First-Line-Drug-Resistant Mycobacteria from Sputum of Pulmonary Tuberculosis Patients
Author(s) -
Imran H. Chowdhury,
Aditi Sen,
Bojlul Bahar,
Avijit Hazra,
Urmita Chakraborty,
Subhadip Choudhuri,
Avranil Goswami,
Nishith Kumar Pal,
Basudev Bhattacharya
Publication year - 2012
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.06093-11
Subject(s) - sputum , tuberculosis , mycobacterium tuberculosis , drug resistance , multiplex polymerase chain reaction , restriction fragment length polymorphism , microbiology and biotechnology , biology , multiplex , sputum culture , polymerase chain reaction , virology , medicine , genetics , gene , pathology
Conventional and molecular techniques were applied to detect and characterize drug resistance of mycobacteria in the sputum samples of clinically confirmed tuberculosis. The sensitivities of mycobacterium detection by ZN staining, culture, multiplex PCR, and restriction fragment length polymorphism (RFLP) were 27.7%, 19.9%, 92.9%, and 95.7%, respectively, but all were 100% specific. The conventional and multiple-allele-specific PCR (MAS-PCR) methods enabled establishment of the drug resistance in 19.3% and 86.9% cases, respectively. We demonstrated that molecular techniques have potential in the accurate diagnosis of tuberculosis.
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