Open AccessTrue Microbiota Involved in Chronic Lung Infection of Cystic Fibrosis Patients Found by Culturing and 16S rRNA Gene Analysis
Author(s) -
Vibeke Børsholt Rudkjøbing,
Trine Rolighed Thomsen,
Morten Alhede,
Kasper Nørskov Kragh,
Per Halkjær Nielsen,
Ulla Johansen,
Michael Givskov,
Niels Høiby,
Thomas Bjarnsholt
Publication year - 2011
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.06092-11
Subject(s) - cystic fibrosis , 16s ribosomal rna , microbiology and biotechnology , biology , lung , gene , ribosomal rna , lung infection , pathology , medicine , genetics
Patients suffering from cystic fibrosis (CF) develop chronic lung infection. In this study, we investigated the microorganisms present in transplanted CF lungs (n = 5) by standard culturing and 16S rRNA gene analysis. A correspondence between culturing and the molecular methods was observed. In conclusion, standard culturing seems reliable for the identification of the dominating pathogens.
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