Open AccessNovel Microsphere-Based Method for Detection and Typing of 46 Mucosal Human Papillomavirus Types
Author(s) -
Vanessa Zubach,
Gerry Smart,
Samuel Ratnam,
Alberto Severini
Publication year - 2011
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.06090-11
Subject(s) - genotyping , typing , human papillomavirus , microsphere , biology , nucleic acid thermodynamics , virology , dna–dna hybridization , in situ hybridization , dna , microbiology and biotechnology , genotype , gene , genetics , base sequence , medicine , engineering , chemical engineering , gene expression
We have developed a novel microsphere-based genotyping method for 46 mucosal human papillomavirus (HPV) types. HPV DNA was amplified by PCR using general primers and typed by hybridization to HPV type-specific probes coupled to sortable microspheres based on the Luminex xMAP technology. Hybridization to each probe was specific for each HPV type without cross-hybridization and sensitive enough to allow typing of HPV contained in clinical specimens. The method was validated with direct sequencing and the Roche Linear Array genotyping method.
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