Use of a Commercial PCR-Based Line Blot Method for Identification of Bacterial Pathogens and the mecA and van Genes from BacTAlert Blood Culture Bottles

In this study, the PCR-based DNA strip assay GenoType BC for the identification of bacteria and the resistance genesmecA ,vanA ,vanB ,vanC1 , andvanC2/3 directly from positive BacTAlert blood culture bottles was evaluated in a multicenter study. Of a total of 511 positive blood cultures, correct identification percentages for Gram-negative bacteria, Gram-positive bacteria, and themecA gene were 96.1%, 89.9%, and 92.9%, respectively. Results were available 4 h after growth detection.