Open AccessSpecies Identification of Clinical Isolates of Anaerobic Bacteria: a Comparison of Two Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Systems
Author(s) -
Ulrik Stenz Justesen,
Anette Holm,
Elisa Knudsen,
Line Bisgaard Andersen,
Thøger Gorm Jensen,
Michael Kemp,
Marianne Nielsine Skov,
Bente Gahrn-Hansen,
Jens Kjølseth Møller
Publication year - 2011
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.05788-11
Subject(s) - mass spectrometry , anaerobic bacteria , bacteria , identification (biology) , chromatography , matrix (chemical analysis) , microbiology and biotechnology , matrix assisted laser desorption/ionization , desorption , biology , chemistry , analytical chemistry (journal) , ecology , genetics , adsorption , organic chemistry
We compared two matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) systems (Shimadzu/SARAMIS and Bruker) on a collection of consecutive clinically important anaerobic bacteria (n = 290). The Bruker system had more correct identifications to the species level (67.2% versus 49.0%), but also more incorrect identifications (7.9% versus 1.4%). The system databases need to be optimized to increase identification levels. However, MALDI-TOF MS in its present version seems to be a fast and inexpensive method for identification of most clinically important anaerobic bacteria.
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