Open AccessVariola Virus-Specific Diagnostic Assays: Characterization, Sensitivity, and Specificity
Author(s) -
Ashley V. Kondas,
Victoria A. Olson,
Yu Li,
Jason Abel,
Miriam T. Laker,
Laura J. Rose,
Kimberly Wilkins,
Jonathan Turner,
Richard L. Kline,
Inger K. Damon
Publication year - 2015
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.03613-14
Subject(s) - cowpox , virology , variola virus , poxviridae , biology , cowpox virus , smallpox , monkeypox , vaccinia , vaccination , recombinant dna , genetics , gene
A public health response relies upon rapid and reliable confirmation of disease by diagnostic assays. Here, we detail the design and validation of two variola virus-specific real-time PCR assays, since previous assays cross-reacted with newly identified cowpox viruses. The assay specificity must continually be reassessed as other closely related viruses are identified.
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