Open AccessComparison of Multiple-Locus Variable-Number Tandem-Repeat Analysis with Pulsed-Field Gel Electrophoresis Typing of Acinetobacter baumannii in China
Author(s) -
Yuan Hu,
Boqing Li,
Dazhi Jin,
Zhigang Cui,
Xiaoxia Tao,
Binghua Zhang,
Jianzhong Zhang
Publication year - 2013
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.03108-12
Subject(s) - multiple loci vntr analysis , variable number tandem repeat , pulsed field gel electrophoresis , acinetobacter baumannii , typing , biology , dna profiling , genetics , microbiology and biotechnology , genotype , gene , bacteria , dna , pseudomonas aeruginosa
A panel of seven variable-number tandem-repeat (VNTR) markers was selected for Acinetobacter baumannii typing analysis (MLVA-7). Compared with pulsed-field gel electrophoresis (PFGE), MLVA-7 provided greater discrimination. We modified the criteria for MLVA complex assignments proposed previously, and a remarkable congruence between MLVA-7- and PFGE-based strain clustering was observed.
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