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High-resolution melt (HRM) analysis can identify sequence polymorphisms by comparing the melting curves of amplicons generated by real-time PCR amplification. We describe the application of this technique to identify Mycobacterium avium subspecies paratuberculosis types I, II, and III. The HRM approach was based on type-specific nucleotide sequences in MAP1506, a member of the PPE (proline-proline-glutamic acid) gene family.

journal of clinical microbiology

Rapid Identification and Differentiation of <i>Mycobacterium avium</i> Subspecies <i>paratuberculosis</i> Types by Use of Real-Time PCR and High-Resolution Melt Analysis of the MAP1506 Locus

Rapid Identification and Differentiation of Mycobacterium avium Subspecies paratuberculosis Types by Use of Real-Time PCR and High-Resolution Melt Analysis of the MAP1506 Locus